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Welcome to Part-6 of the Whole Genome Sequencing & Assembly Workflow | From Zero to Hero series. In this session, we will focus on interpreting Unicycler output and performing plasmid identification using Plassembler.

Understanding Unicycler results and identifying plasmids correctly is a critical step in bacterial genome assembly, especially for studies related to antimicrobial resistance, horizontal gene transfer, and mobile genetic elements.

In this session, you will learn:
• How to understand and interpret Unicycler assembly output
• Difference between chromosomal contigs and plasmid contigs
• Introduction to Plassembler for plasmid detection
• Running Plasmid Finder using Plassembler
• Identifying plasmid-associated sequences from assembled genomes
• Practical relevance of plasmid detection in genomics and bioinformatics research

This session is part of Bioinformatics Ka Chilla on Codanics, where we cover real-world genome assembly workflows step by step using practical tools and datasets.