Unit 1 Part 1 | Instrumental Methods of Analysis (IMA) · B Pharm 7th Semester (earlier PCI/CBCS syllabus)
Learn ultraviolet (UV) visible spectroscopy in instrumental methods of analysis (IMA) Unit 1 for B Pharm 7th semester.

Covered topics
Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations. Instrumentation- Sources of radiation, wavelength selectors, sample cells, detectors: Photo tube, Photomultiplier tube, Photovoltaic cell, Silicon Photodiode.
Applications- Spectrophotometric titrations, single-component and multi-component
analysis

Study this subject
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Next lesson: Fluorimetry
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